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Anti Surfactant protein A (human, hSP-A), biotinylated

Mouse monoclonal antibody

Cat.No. HYB 238-04B

Subclass IgG1/k

Presentation:

Preparation: Biotinylated
Content: Available in 100 無
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.13 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8慢

Antigen:

Surfactant protein A (SP-A) is synthesized and secreted by lung epithelial cells. It belongs to group III of the family of C-type lectins and members of this group has overall structure consisting of multiple globular head regions linked by triple-helical, collagen-like, strands. This group also includes SP-D and the serum proteins mannan-binding protein, conglutinin and collectin-43, all of which have been shown to bind to the C1q receptor found on a wide variety of cells. Both SP-D and SP-A have been shown to enhance oxygen radical production by alveolar macrophages. The serum concentration is 45 ng/ml in healthy individuals (1).

Immunogen:

Native SP-A purified from bronchioaveolar lavage from proteinosis patients

Specificity:

HYB 238-04 is specific for human pulmonary SP-A

Epitope specificity:

Not determined

Reactivity:

In Western blotting after SDS-PAGE HYB 238-04 reacts with reduced forms of SP-A and weaker with non-reduced forms (3).
In immunohistochemical staining from paraffin embedded tissue samples, HYB 238-04 reacts specifically with human SP-A. Immunoreactivity is seen with alveolar type II cells, nonciliated bronchial cells and a subset of alveolar macrophages. No reactivity in samples from pancreas, intestines, thymus, prostate gland or the peritoneal cavity lining cells (3).
HYB 238-04 reacts strongly with SP-A when tested in ELISA with SP-A directly coated onto the microtiter well.

Application:

Method	Usability	Dilution guideline	References
ELISA	Yes
Immunoblotting	Yes		3
Immunohistochemistry	Yes		1, 3

Application Remarks:

References:

1. Holmskov UL (2000) Collectins and collectin receptors in innate immunity. APMIS Suppl 100:1-59.
2. Johansson J, Curstedt T (1997) Molecular structures and interactions of pulmonary surfactant components. Eur J Biochem 244:675-693.
3. Madsen J, Tornoe I, Nielsen O, Koch C, Steinhilber W, Holmskov U (2003) Expression and localization of lung surfactant protein A in human tissues. Am J Respir Cell Mol Biol 29:591-7.